Skip to main content
. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Science. 2012 Feb 16;335(6073):1195–1200. doi: 10.1126/science.1216557

Fig. 3. Ectopic Lin28 expression in adult BM HSPCs confers fetal-like B cell development.

Fig. 3

Plots depict flow cytometric analyses of (A) CD19+ peritoneal cavity (PerC) and (C) B220+ splenic (SPL) B-cell subsets in recipients of adult BM HSPCs transduced with the indicated retroviral particles. (B) Graph shows percentages of B-2 (CD19+ B220hi CD5−ve), B-1a (CD19+ B220lo CD5+) and B-1b (CD19+ B220lo CD5−ve) B-lymphocytes among the GFP+ and GFP−ve fractions of peritoneal cavity CD19+ B cells in eight independent BM chimeras. (D) Graph shows percentages of marginal zone (MZ) (B220+ CD1d+ CD23−ve) and follicular B-2 (FoB) cells (B220+ CD1d−ve CD23+) among GFP+ and GFP−ve splenic CD19+ B cells in six independent BM chimeras. (E) FACS analysis show the presence of B-cells in the peritoneal cavity and spleen of BM chimeras reconstituted with Lin28-RV transduced Il7rα−/−BM HSPCs. Data are representative of two independent BM chimeras. ***P value<0.0001 represent statistical significance calculated by t-test. n.s indicates not statistically significant.