Table 2.

Crystal structures of Kapβ2, Csel complexes and a Crml fragment

Structure	PDB-ID	Ref.	Organism	Resolution (Å)	Protein constructs in crystals	Residues in model	Domains/ motifs	Contact residues (mol. 1)	Contact residues (mol. 2)	Contact residues (mol. 3)	Contact type	Disruptive interface mutants
Kapβ2-	1QBK	[11]	β2: human	3.0	β2: 1–890	β2:	β2:	β2:	Ran:
RanGppnhp			Ran: human		Ran: 1–216	3–152, 158–166,	HEAT repeats	I114	R110		HP*
						170–890		S165	R110		Polar
								E275	K141		Polar
						Ran:		E278	K141		Polar
						8–197		I315	P172		HP*
								E332	N154		Polar
								R336	D148		Polar
								P337	Y155		HP*
								W373	N143		Polar
								E682	K127		Polar
Kapβ2-M9NLS	2H4M	[12]	β2: human	3.1	β2: 1–336-	β2:	β2:	β2:	M9NLS:			β2:
					GGSGGSG-	6–36,	HEAT repeats	R805	N268		Polar	W460A/
					368–890	44–77,		K765	Q269		Polar	W730A
						57–73,		E809,I773	F273		HP*
			M9NLS: from		M9NLS: 257–305	80–319,	M9NLS:	W730	P275		HP*
			hnRNP Al, human			368–890	extended	T766	M276		HP*
								T547	N280		Polar	M9NLS:
						M9NLS:		F584,E588,	F281		HP*	G274A/
						257–305		V643			HP*	P288A/
								A505,T506,	R284		Polar	Y289A
								E509,D543,			Polar
								T547			Polar
								L419,I457,	P288		HP*
								W460			HP*
								A380,A381,	Y289		HP*
								D384,L419,			HP*
								R464			Polar
Csel	1Z3H	[13]	Yeast	3.1	1–959	Chain A: 2–529,	HEAT repeats
						544–870, 892–959
						Chain B: 2–156,
						162–525, 545–
						875, 896–959
Csel-Kap60p-	IWA5	[14]	Csel: yeast	2.0	Csel: 1–960	Csel:	Csel:	Csel:	Kap60p:	Ran:		Ran:
Ran						1–193, 196–248,	HEAT repeats	R159	E42		Polar	R76A/
						255–371, 376–		F164	V41		HP*	D77A
						413, 418–880,		F164	L43		HP*
						886–958		E166	R17		Polar
			Kap60p: yeast		Kap60p:	Kap60p: 12–19,	Kap60p: IBB,	I167	L43		HP*
					1–530	31–58,	armadillo	D220	R44		Polar	K37D/
						87–513	domain	D227	K45		Polar	K152A
								D79	R44		Polar
			Ran: canine		Ran: 1–176	Ran:		R321	D435		Polar
						7–176		E387	K431		Polar	R95D/
								R579	E514		Polar	K99A/
								K62		R76	Polar	K130A/
								N63		D77	Polar	K134A
								N609		K152	Polar
								D653		K37	Polar
								E656		K152	Polar
								H894		T93	Polar
								H894		D128	Polar
									E484	KI34	Polar
									Q490	K95	Polar
									N492	K99	Polar
									Y498	K130	Polar
									E506	K130	Polar
									E511	K132	Polar
Crml	1W9C	[15]	Human	2.3	707–1034	707–1027	HEAT repeats