Figure 10.
Effect of changing the closed-system intracellular buffering power β on the surface (pHS) and intracellular pH (pHi) time profiles. We show the results of six simulations corresponding to β values of 1000·βstd, 5·βstd, 2·βstd, βstd, βstd/2, and 0.·βstd is the buffering power used for the “standard in-silico experiment” (i.e., the β for a single buffer with pK = 7.1 and concentration ≈ 27.3126 mM). The curve for β = 1000·βstd is an approximation of an infinite β. (A) Time course of simulated surface pH. (B) Time course of simulated intracellular pH at a depth of ≈ 50 μm. Note the break in the ordinate, in this panel, which is necessary for showing the large pHi decrease that would occur with a β of 0.