Figure 6.

AV synthesis is not primarily affected in sPD cybrid cells. (A) LC3B immunoblot for autophagosome synthesis assessment in CT and sPD cybrids treated with or without lysosomal inhibitors (NL) at three different time points (2, 4 and 6 h) (n =4). (B) Time course for autophagic vacuole (AV) levels. AV levels correspond to the densitometry values of LC3-II for each condition at each time point (n =4, ***P< 0.001, versus CT cybrids). (C) Assessment of autophagic synthesis. Rates of AV synthesis were determined by comparing LC3-II levels at two different time points after the addition of NL (4 versus 2 and 6 versus 4 h) (n =4).