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. 2012 Jul 27;21(21):4680–4702. doi: 10.1093/hmg/dds309

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© The Author 2012. Published by Oxford University Press

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Autophagic induction is not compromised in sPD cybrid cells. (A) Immunoblot for Beclin-1 from CT and sPD cybrids after culture in serum (+) or serum (−) conditions and treatment with or without NL. (B) Densitometric analysis of endogenous levels of Beclin-1 (n= 8, ***P< 0.001, versus S+ CT cybrids). (C) Immunoblot for Bcl-2 from CT and sPD cybrids after culture in serum (+) or serum (−) conditions and treatment with NL. (D) Densitometric analysis of endogenous levels of Bcl-2 (n= 6, *P< 0.05, **P< 0.01, ***P< 0.001, versus S+ CT cybrids). (E) Representative immunoblots for Bcl-2 and Beclin-1 cellular subcompartmentalization in mitochondria (M)- and cytosol (C)-enriched fractions. (F) and (G) Densitometric analysis of the levels of Bcl-2 (F) (n= 3, **P< 0.01, versus S+ CT cybrids; ^##P< 0.01, versus S− CT cybrids) and Beclin-1 (G) (n= 3). (H) Co-immunoprecipitation of Beclin-1 and Bcl-2 in CT and sPD cybrids maintained in serum (+) or serum (−) conditions. Levels of Beclin-1 (top) and Bcl-2 (bottom) in the input, immunoprecipitate (IP) and flow through (FT) are shown. (I) Determination Bcl-2/Beclin-1 physical interaction (n= 3).