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Enumeration of circulating TFH cells in whole blood. TFH cells were counted in whole blood using four different phenotypic definitions. (a)–(d) Representative gating strategy. (e) CD4+/CXCR5+; (f) CD4+/CD45RO+/CXCR5+; (g) CD4+/CXCR5+/PD-1hi; (h) CD4+/CXCR5+/ICOShi. No differences were detected between patients with RA and controls (P ≥ 0.4 in all cases). Spots represent individual patients. Mean ± SEM are shown.
