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. 2012 Oct 3;103(7):1500–1509. doi: 10.1016/j.bpj.2012.08.051

Figure 1.

Figure 1

(a) TIRF image of background fluorescence (taken with supported POPC/POPG lipid membrane only). (b) TIRF image of HiLyte Fluor 488 β-amyloid (1–40) peptide bound to POPC/POPG lipid membrane. The concentration of β-amyloid (1–40) peptide in solution is 100 nM. (c) The kinetics of the same concentration of β-amyloid (1–40) peptide binding to the membrane. The data could be fit to a single exponential very well, indicating a first-order binding kinetics.