Figure 3.
Free-energy landscapes of various ANK and TPR systems projected along Rg and Q. The free energy of the system with a given Rg and a given number of contacts (Q, normalized by the total number of contacts in the native state) is shown; dark-blue regions indicate relatively populated conformations (i.e., low free energy). Top panels: In the ANK system, the appearance of two dark-blue regions, which is indicative of two relatively populated systems (unmodified ANK5, left), indicates that it is becoming a more continuously populated region in the reduced interface system (ANK5 with intercontacts whose strength is reduced by 50% (εinter = 0.5; right). Bottom panels: In the TPR system, there is only one continuous dark-blue region, indicative of a population of intermediates (unmodified TPR 10 and TPR with intercontacts with 50% increased strength (εinter = 1.5), left and right panels, respectively).