Table 1.

Results from the linear fits of the ln k versus force for constant-force-feedback and constant-trap-position experiments

Molecule	Δx‡Unfolding (nm)∗	Δx‡Folding (nm)∗	ΔxTotal (sum) (nm)∗	ΔxTotal (measured) (nm)†	Ratio of ΔxTotal‡	ln(kCoincident)∗
Constant-force feedback (partition method, 100 Hz)
DNA hairpin	11.3 ± 0.6	12.1 ± 1.1	23.5 ± 1.6	17.7 ± 0.4	1.33	1.3 ± 0.4
RNA hairpin	12.1 ± 2.5	13.9 ± 2.6	26.0 ± 3.2	19.2 ± 0.4	1.35	0.9 ± 0.4
Protein	14.8 ± 8.1	22.4 ± 6.4	37.2 ± 5.6	18.9 ± 0.4	1.97	2.0 ± 0.6
Constant-trap position (partition method, 100 Hz)
DNA hairpin	9.3 ± 1.4	10.9 ± 1.8	20.2 ± 1.5	17.7 ± 0.4	1.14	1.3 ± 0.6
RNA hairpin	8.9 ± 5.6	13.2 ± 3.6	22.1 ± 6.6	19.2 ± 0.4	1.15	0.9 ± 0.9
Protein	7.5 ± 3.1	15.9 ± 5.1	23.4 ± 6.0	18.9 ± 0.4	1.24	2.4 ± 0.7
Constant-trap position (BHMM method, 1000 Hz)
DNA hairpin	7.8 ± 0.7	10.2 ± 0.9	18.0 ± 1.3	17.7 ± 0.4	1.02	1.7 ± 0.5
RNA hairpin	7.9 ± 1.9	11.6 ± 2.1	19.5 ± 3.1	19.2 ± 0.4	1.02	1.5 ± 0.7
Protein	6.1 ± 1.1	14.4 ± 3.9	20.5 ± 3.2	18.9 ± 0.4	1.09	2.8 ± 0.7

^∗Average values reported with a 95% CI.

^†Distance determined from fitting a histogram of the trap position from a constant-force-feedback experiment with two Gaussian distributions and determining the difference between the two Gaussian means with a 95% CI.

^‡Ratio of the calculated sum of the distances to the transition state to the experimentally measured distance between the two states.