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Fluvastatin alters FLT3/ITD signaling. (A) BaF3 FLT3/ITD cells were treated with 1μM fluvastatin for the indicated times and 50 μg of lysate from each time point was analyzed by SDS-PAGE and Western blotting. (B) Cells were treated with 1μM fluvastatin for 24 hours and lysates were immunoprecipitated with a FLT3 antibody (S-18). Blots were then probed for FLT3, phosphotyrosine, c-cbl, or phospho c-cbl. Typical results of 3 experiments are shown.
