Figure 5.
Inhibition of seizure protection by the A1-specific antagonist DPCPX. Grafting of ADA-O capsules into the lateral brain ventricles of kindled rats was followed by complete protection from seizures (mean seizure grade: 0.2 ± 0.3 as determined 4 and 9 days after grafting; n = 3 rats). Administration of DPCPX (1 mg/kg, i.p.) at days 5 and 10, respectively, resulted in a near-complete restoration of tonic–clonic seizure activity (grade 4.7 ± 0.3, mean value from both days) as tested 20 min after drug injection. The effect of DPCPX was transient, being strongly reduced 3 days after the injection (mean seizure grade: 0.9 ± 0.7). The values given are the means of the seizure grades measured at day 5 plus day 10 (n = 6). (Bars, ±SEM.)