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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Mol Microbiol. 2006 May;60(3):630–643. doi: 10.1111/j.1365-2958.2006.05135.x

Fig. 5.

Fig. 5

FlgM is unstable in the absence of Flk.

A. Immunoblot analysis of FlgM protein at different time points following the addition of the translation inhibitor spectinomycin. Cellular FlgM turns over more rapidly in a strain with a functional hook-basal body (TH4803, HBB+) because of FlgM secretion. In a strain deleted for the P- and l-rings (TH7025, Ring−), FlgM accumulates in the cell and is relatively stable. A null mutation in flk (TH7026) results in a sharp decrease in FlgM stability in the ring mutant strain.

B. A graph representation of the data in (A).