Skip to main content
. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Oncogene. 2007 Sep 10;27(10):1412–1420. doi: 10.1038/sj.onc.1210784

Figure 2.

Figure 2

A C-terminal element determines dUTPase and E4-ORF1 trimerization. (a) C-terminal mutants of human dUTPase and E4-ORF1. Gray shaded regions indicate residues needed for trimer formation. Substitution mutations are indicated in bold. E4-ORF1 PBM residues are underlined. (b) β8 mutations abolish E4-ORF1 trimer formation. 293 T cells were transfected with pGW1 encoding the indicated mutant E4-ORF1. Extracts in RIPA buffer were fractionated by SEC with elution buffer containing 0.1% DOC and blotted with E4-ORF1 antibody.