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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Oncogene. 2007 Sep 10;27(10):1412–1420. doi: 10.1038/sj.onc.1210784

Figure 4.

Figure 4

Residues KI in the TRI element additionally control E4-ORFl binding to ZO-2 and Dlg1. (a) E4-ORF1 TRI element mutants have trimerization (TRI)-independent defects in binding ZO-2. 293 cells were co-transfected with pGW1 encoding HA-ZO-2 and either pGW1 (vector) or pGW1 encoding wt or the indicated mutant E4-ORF1. Extracts in RIPA buffer were IP’d with HA antibody. Recovered proteins and total cell extracts (Input) were blotted with HA or E4-ORF1 antibody. (b) E4-ORF1 TRI element mutants have TRI-independent defects in binding Dlg1. Identical experiments as those described in (a) were conducted, except pGW1 encoding HA-Dlg1 was used.