Skip to main content
. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Oncogene. 2007 Sep 10;27(10):1412–1420. doi: 10.1038/sj.onc.1210784

Figure 5.

Figure 5

E4-ORF1 trimers bind Dlg1. (a) C-terminal sequence of E4-ORF1 mutants and their capacities to trimerize and bind Dlg1. Results summarized from Figure 5b, Supplementary Figure S10, or unpublished data. The TRI or expanded PBM element are gray shaded or underlined, respectively. Inserted residues are italicized. Alanine substitutions are indicated in bold. (b) Monomeric AAA + KI fails to bind endogenous Dlg1. CREF cells were transduced with pBABE (vector) or pBABE encoding wt or the indicated mutant E4-ORF1. Extracts in RIPA buffer were IP’d with E4-ORF1 antibody. Recovered proteins were blotted with Dlg1 or E4-ORF1 antibody. Left and right panels represent two independent experiments. (c) E4-ORF1 trimers bind the Dlg1 PDZ1 + 2 tandem. 293 T cells were transfected with pGW1 encoding wt E4-ORF1 or HA-Dlg1 PDZ1 + 2 alone or in combination. Extracts in RIPA buffer were fractionated by SEC with elution buffer containing 0.1% DOC and blotted with E4-ORF1 or HA antibody.