TABLE 1.
List of primers
Vector | Gene | RE site | Primer sequence |
---|---|---|---|
pcDNA6/v5 (Invitrogen) | NSP4 | EcoRI | 5′-GAATTCATGGAAAAGCTTACCGACC-3′ |
EcoRV | 5′-GATATCCACATTGCTGCAGTCACTTCT-3′ | ||
pcDNA6/v5 (Invitrogen) | VDAC1 | EcoRI | 5′-GATATCATGGCTGTGCCACCC-3′ |
XhoI | 5′-CTCGAGCGTGCTTGAAATTCC-3′ | ||
pcDNA6/v5 (Invitrogen) | ANT3 | EcoRI | 5′-GAATTCTATGACGGAACAGGC-3′ |
EcoRV | 5′-GATATCGATCACCTTCTTGAG-3′ | ||
pAcGFP1-C2 (Clontech) | NSP4 | EcoRI | 5′-GAATTCATGGAAAAGCTTACCGACC-3′ |
SalI | 5′-GTCGACCACATTGCTGCAGTCACTTCT-3′ | ||
pAcGFP1-C2 (Clontech) | NSP4 Δ1–25 | EcoRI | 5′-GAATTCTG GAGGATCCAGGAATGGC-3′ |
SalI | 5′-GTCGACCACATTGCTGCAGTCACTTCT-3′ | ||
pAcGFP1-C2 (Clontech) | NSP4 Δ1–48 | EcoRI | 5′-GAATTCTGGCATCCATTCCAACAATG-3′ |
SalI | 5′-GTCGACCACATTGCTGCAGTCACTTCT-3′ | ||
pAcGFP1-C2 (Clontech) | NSP4 Δ1–60 | EcoRI | 5′-GAATTCTGTCAAAATGTTCATATAAAG-3′ |
SalI | 5′-GTCGACCACATTGCTGCAGTCACTTCT-3′ | ||
pAcGFP1-C2 (Clontech) | NSP4 Δ1–83 | EcoRI | 5′-GAATTCTGGGTTATAAAGAGCAGATAAC-3′ |
SalI | 5′-GTCGACCACATTGCTGCAGTCACTTCT-3′ | ||
pAcGFP1-C2 (Clontech) | NSP4 Δ143–175 | EcoR1 | 5′-GAATTCATGGAAAAGCTTACCGACC-3′ |
SalI | 5′-GTCGACTATTTCGCCTGTCGTTTGCAC-3′ | ||
pAcGFP1-C2 (Clontech) | NSP4 Δ92–175 | EcoRI | 5′-GAATTCATGGAAAAGCTTACCGACC-3′ |
SalI | 5′-GTCGACAGTAGTTATCTGCTCTTTATAA-3′ | ||
pAcGFP1-C2 (Clontech) | NSP4 Δ74–175 | EcoRI | 5′-GAATTCATGGAAAAGCTTACCGACC-3′ |
SalI | 5′-GTCGACCTTTATATGAACATTTTGAC-3′ | ||
pAcGFP1-C2 (Clontech) | NSP4 (61–91) | EcoRI | 5′-GAATTCTGTCAAAATGTTCATATAAAG-3′ |
SalI | 5′-GTCGACAGTAGTTATCTGCTCTTTATA-3′ | ||
pAcGFP1-C2 (Clontech) | NSP4 (61–83) | EcoRI | 5′-GAATTCTGTCAAAATGTTCATATAAAG-3′ |
SalI | 5′-GTCGACTGCCAATTTTAACAACGTATTA-3′ | ||
pAcGFP1-C2 (Clontech) | NSP4 (74–91) | EcoRI | 5′-GAATTCACAATTTTTATACGTTGTTAAA-3′ |
SalI | 5′-GTCGACAGTAGTTATCTGCTCTTTATAA-3′ | ||
pFLAG-CMV6 (Sigma) | NSP4 | EcoRI | 5′-GAATTCAATGGAAAAGCTTACCGACC-3′ |
EcoRV | 5′-GATATCCACATTGCTGCAGTCACTTCT-3′ |