FIGURE 11.

Loss of GRX3/GRX4 affects the low iron transcriptional response but does not directly affect the high iron transcriptional response. A and B, wild type and Δgrx3/grx4 cells were transformed with either a FET3-lacZ reporter construct (A) or a CCC1-lacZ reporter construct (B). The cells were grown overnight in CM, in CM made iron-limited by the addition of 80 μm BPS, or in high iron (500 μm). Cells were homogenized, and β-galactosidase activity and cell protein determined. The data are presented as specific activity normalized to cell protein, and the error bars reflect the S.D. of three independent experiments.