FIGURE 12.

Increased oxidants do not suppress Yap5-induced expression of CCC1. A, wild type, Δglr1, and Δtrr1 cells were transformed with a CCC1-lacZ plasmid. Cells were incubated in low iron medium, and then 500 μm iron was added. After a further 2-h incubation, cells were harvested, and β-galactosidase and cell protein were determined. B, cells (Δccc1, Δccc1Δisu1, Δccc1Δssq1 transformed with MET3-SSQ1, and Δccc1Δyfh1 transformed with MET3-YFH1) were transformed with CCC1-lacZ. Expression of MET3-SSQ1 and MET3-YFH1 was controlled by the presence (off) (10×) or absence (on) of methionine. Cells were grown in low iron medium anaerobically overnight, and 500 μm iron was added for an additional 2.5 h under anaerobic conditions. Cells were harvested, and β-galactosidase activity and cell protein were determined. The data are presented as specific activity normalized to cell protein, and the error bars reflect the S.D. of three independent experiments.