FIGURE 7.
Loss of ISU1 prevents iron-dependent induction of CCC1-lacZ. Δccc1 or Δccc1Δisu1 cells were transformed with a GDO plasmid and either FET3-lacZ or CCC1-lacZ reporter constructs. Cells were grown overnight in CM medium (CM) or CM medium with 80 μm BPS plus (CM+Fe) or minus 100 μm FeSO4 (CM+BPS). Cells were homogenized, and FET3-lacZ (A), GDO (B), and CCC1-lacZ (C) activities and cell protein were determined. The data are presented as specific activity, and the error bars reflect the S.D. of three independent experiments.