FIGURE 9.

Mutations in Fe-S cluster synthesis genes lead to decreased transcripts for Yap5 targets. Total RNA was isolated from cells grown in an iron-replete medium. A, Δssq1 cells containing a MET3-regulated SSQ1-expressing plasmid were grown in a medium without methionine (on) or with 10× methionine (off) overnight. CCC1 mRNA and FET3 mRNA were measured by real-time PCR. B, Δccc1Δssq1 cells containing a MET3-regulated SSQ1-expressing plasmid or Δccc1Δyfh1 cells with a MET3-regulated YFH1-expressing plasmid were grown in a medium without methionine (on) or with 10× methionine (off) overnight. Δccc1 and Δccc1Δisu1 were grown in an iron-replete medium overnight. Real-time PCR was performed for TYW1 transcription (a Yap5-regulated gene). -Fold changes of each gene transcriptional level were quantified based on CMD1 as an internal control. The error bars reflect the S.D. of two independent experiments.