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. 2012 Jul 30;287(42):35646–35657. doi: 10.1074/jbc.M112.377481

FIGURE 1.

FIGURE 1.

Hsf1-deficient cells exhibit reduced numbers of mammary gland tumors. A, Kaplan-Meier tumor-free survival (%) in days is presented for Hsf1+/+Neu+ (n = 27), Hsf1+/−-Her2/Neu+ (n = 30), and Hsf1−/−Neu+ (n = 13) mice. p values for Hsf1−/−Neu+ versus Hsf1+/+Neu+ and Hsf1+/−Neu+ was <0.0006 using log-rank test. B, upper panel, representative H&E-stained tumor tissue sections extracted from the indicated genotype. Lower panel, representative H&E-stained section of lung tumor metastases extracted from the indicated genotypes. Bars, 100 μm. C, Ki-67 immunohistochemical staining of tumor tissue sections of the indicated genotypes. Bar, 50 μm. Red color indicates Ki-67 (arrowheads), and blue color indicates DAPI (nuclei) immunostaining. Quantification of Ki-67-positive cells between the indicated genotypes is presented in the right panel. Bars are means ± S.D. (n = 3 mice). Statistical significance is as follows: *, p < 0.05. D, TUNEL assays of the tumor tissue sections of the indicated genotypes. Arrowheads show some of the TUNEL-positive cells. Bar, 50 μm. E, CD31 immunohistochemical staining of tumor tissue sections of the indicated genotypes. Arrowheads show some of the CD31 positive cells. Bar, 50 μm. Quantification of the CD-31-positive cells in tumor tissue sections of the indicated genotypes is shown in the right panel. Bars are means ± S.D. (n = 3 mice, with the exception of Hsf1−/−Neu+ mice that only generated one tumor). Statistical significance is as follows: **, p < 0.01; ***, p < 0.001.