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. 2012 Jul 30;287(42):35646–35657. doi: 10.1074/jbc.M112.377481

FIGURE 3.

FIGURE 3.

Whole-mount preparation and histological analyses of mammary glands from mice with or without Hsf1 gene or Neu oncogene. A–C, whole-mount preparation of number 4 mammary glands from 8- to 10-week-old virgin Hsf1+/+, Hsf1+/−, and Hsf1−/− female mice, without Neu (A), or with Neu (B) expression. Arrowheads indicate branches and terminal end buds. Quantification of the lateral side branches in the three genotypes is indicated in C. Bars are mean ± S.D. Magnification is ×50. n = 3 mice. Statistical significance is as follows: **, p < 0.01 and ***, p < 0.001. D, H&E-stained cross-sections of mammary glands from the indicated genotypes. Bar, 50 μm. Arrowheads indicate tubules and branches. E and F, immunohistochemical staining of cross-sections of mammary glands from the indicated genotypes using αSMA (E) or keratin 18 (F) as the primary antibody. Arrowheads indicate some of the positively stained myoepithelial (E) or luminal epithelial cells (F) cells. Bar, 10 μm.