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Syndecan-1 transmembrane domain slows focal adhesion disassembly in migrating lung epithelial cells. Cells stably expressing paxillin-eGFP were injured, and the turnover of focal adhesions in migrating cells was evaluated. A, kymographs of a focal adhesion at the leading edge of a migrating cell. Horizontal line = 10 min; vertical line = 5 μm. B, the normalized intensity of the focal adhesion in A was measured and plotted over time.
