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Syndecan-1 transmembrane domain attenuates focal adhesion disassembly after nocodazole washout. Cells stably expressing paxillin-eGFP were treated with nocodazole (10 μm, 4 h), and the disassembly of focal adhesions was evaluated after washout. A–E, focal adhesion disassembly was observed in B2bshRNA.scr cells, B2bshRNA.hSdc1 cells, and B2bshRNA.hSdc1 cells expressing wild-type and mutant mouse syndecan-1. F, intensity curves of the focal adhesions in A–E.
