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Syndecan-1 transmembrane domain facilitates recovery of fluorescence after photobleaching in migrating lung epithelial cells. Cells stably expressing paxillin-eGFP were injured, and the focal adhesion at the leading edge of the migrating cells was selectively photobleached. A, focal adhesion prior to (−1 s) and after photobleaching. B, normalized intensity of the representative focal adhesion in A plotted over time.
