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. 2012 Aug 23;287(42):34917–34926. doi: 10.1074/jbc.M112.359356

TABLE 2.

Steady-state kinetic parameters of MOF and deacetylated MOF

Enzyme Substrate appkcat appKm appkcat/appKm
min1 μm min1·μm1
MOF H4–20 37.75 ± 0.63 788.8 ± 27.4 0.048 ± 0.002
AcCoA 14.28 ± 0.45 0.55 ± 0.07 25.96 ± 3.45
MOFdeac H4–20 22.20 ± 0.79 897.7 ± 63.9 0.025 ± 0.002
AcCoA 8.55 ± 0.40 0.47 ± 0.09 18.19 ± 3.73