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. 2012 Aug 23;287(42):35192–35200. doi: 10.1074/jbc.M111.322032

FIGURE 5.

FIGURE 5.

Transfection of GT1-7 neurons with NR1-NR2a is neuroprotective. A, compared with non-transfected control cells, transfection resulted in a selective increase in both NR1 and NR2a protein expression in mitochondria demonstrated by Western blotting (quantified in optical density units). B, cell death was reduced by ∼60% in transfected GT1-7 cells 24 h following exposure to l-glutamate compared with either non-transfected cells or cells transfected with mutant NR1-NR2a. C, both control cells and NR1-NR2a-transfected cells had equivalent levels of mitochondrial calcium following a 30-min incubation in the absence of l-glutamate. A slight but significant elevation in [Ca2+]m was seen in both non-transfected control cells and cells transfected with mutant NR1-NR2a following a 30-min exposure to l-glutamate compared with untreated control cells. In contrast, exposure of NR1-NR2a-transfected cells to l-glutamate caused a 2-fold increase in [Ca2+]m compared with untreated non-transfected control cells. *, p < 0.01 versus the control; **, p < 0.001 versus the control + l-glutamate. D, ATP levels doubled in GT1-7 cells transfected with NR1-NR2a but not mutant NR1-NR2a after exposure to l-glutamate. *, p < 0.001 versus the control.