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. 2012 Aug 13;287(42):35092–35103. doi: 10.1074/jbc.M112.383737

FIGURE 1.

FIGURE 1.

Curli subunits cross-seeded in vitro. A, normalized ThT fluorescence monitoring the polymerization kinetics of 10 μm E. coli CsgA (CsgAEC) alone (●), or in the presence of 5% (w/w) sonicated CsgAEC seeds (○), S. typhimurium CsgA (CsgAST) seeds (Δ), or C. koseri CsgA (CsgACK) seeds (□). B, 10 μm freshly purified CsgAST polymerized with no seeds (●), or in the presence of 5% sonicated CsgAST seeds (○), CsgAEC seeds (Δ), or CsgACK seeds (□). C, 10 μm CsgAEC polymerized alone (●), or in the presence of 2 (○), 5 (Δ), or 10% (□) S. oneidensis CsgA (CsgASO) seeds. D, 10 μm CsgAEC polymerized alone (●), with 5% CsgBEC seeds (○) or with 5% CsgBST seeds (Δ). E, 10 μm CsgAST polymerized alone (●), with 5% CsgBEC seeds (○) or with 5% CsgBST seeds (Δ).