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. 2012 Aug 15;287(42):35360–35369. doi: 10.1074/jbc.M112.365031

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — In vivo inositol phosphate production and rescue of ipmkΔ. A, HPLC traces of soluble inositol phosphates. HPLC traces of soluble [³H]IP species from ipmkΔ transformed with indicated A. thaliana IPMK constructs. B, Western blot of IPMK construct expression. Soluble A. thaliana IPMK expression detected with α-HA and normalized to α-actin. K117W/K121W and D247A blots are from a second series. C, growth on ornithine. 10-Fold serial dilution spot assays on CSM-Ura, Glu-Ura, or Orn-Ura media. Vect, vector.