FIGURE 1.

Clofibrate and fenofibrate activate PPARα signaling in MCF-7 cells. Cells were transfected with the peroxisome proliferator response element-luciferase reporter and RXRα cDNA constructs and treated with 500 μm clofibrate, 100 μm fenofibrate, and 20 μm troglitazone in the presence or absence of 5 μm GW6471 for 4 h. Cell lysates were prepared, and luciferase activity was assayed. Data (mean ± S.E., n = 3) are expressed as percentages of the luciferase activity detected in untreated control cells. *, p < 0.05 compared with control cells using one-way ANOVA, followed by Dunnett's analysis.