Skip to main content
. 2012 Aug 29;287(42):35161–35169. doi: 10.1074/jbc.M112.367367

FIGURE 5.

FIGURE 5.

Knockdown of pVHL attenuates clofibrate-induced suppression of HIF-1α signaling. A, left panel, MCF-7 cells were transfected with a pool of pVHL siRNAs (50–100 pmol/ml; siRNA-a, siRNA-b, and siRNA-c) for 48 h. Right panel, MCF-7 cells were transfected with individual pVHL siRNAs (100 pmol/ml; siRNA-a or siRNA-b) for 48 h. Cell lysates were prepared, and Western blotting was performed using antibodies against pVHL and β-actin. Shown are representative images of three individual experiments. IB, immunoblot. B, left panel, MCF-7 cells were cotransfected with a pool of pVHL siRNAs (100 pmol/ml) and the pGL3-HRE-luciferase reporter construct. Right panel, MCF-7 cells were cotransfected with pVHL siRNA-b (100 pmol/ml) and the pGL3-HRE-luciferase reporter construct. 48 h after transfection, cells were treated with 500 μm clofibrate for 4 h prior to placement in a hypoxia chamber for 16 h. Cell lysates were prepared, and luciferase activity was assayed. Data (mean ± S.E., n = 3) are expressed as percentages of the luciferase activity detected in untreated cells under normoxia. *, p < 0.05 analyzed by one-way ANOVA analysis.