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. 2012 Aug 25;287(42):34992–35003. doi: 10.1074/jbc.M112.389338

FIGURE 6.

FIGURE 6.

mRNA status of different types of FA desaturase (A) and antioxidant (B) genes in WT, KO, and OE cells. Measurements of gene transcript abundance was analyzed by using quantitative RT-PCR as detailed under ”Experimental Procedures.” ΔSLD, sphingolipid Δ4 desaturase (LmjF.26.1690); Δ12FAD, Δ12 fatty acid desaturase (LmjF.10.0010); Δ12FAD1, Δ12 fatty acid desaturase (LmjF.33.3270); Δ9FAD, Δ9 fatty acid desaturase (LmjF.24.2250); Δ5FAD, Δ5 fatty acid desaturase (LmjF.07.1090); Δ6FAD, Δ6 fatty acid desaturase (LmjF.36.6950); Δ4FAD, Δ4 fatty acid desaturase (LmjF.14.1340). SOD (LmjF.30.2770); APX, ascorbate peroxidase (LmjF.34.0070); GAPDH (LmjF.30.2980); NUOR, NADH-ubiquinone oxidoreductase (LmjF.05.0980); TR, trypanothione reductase (LmjF.05.0350); GPX, non-selenium glutathione peroxidase (LmjF.26.0810); TPX, tryparedoxin peroxidase (LmjF.15.1120). All data were normalized using 18 S rRNA as the endogenous control. Data show the mean ± S.D. of three independent experiments. * p, 0.05, compared with WT sample. All the data are representative of three independent transfection experiments.