TABLE 1.
Catalytic activities of LmNcb5or
The catalytic activity (kcat) is expressed as s−1. NADH oxidation, potassium ferricyanide, DCIP, and cytochrome c reduction rates were determined at 25 °C as described under “Experimental Procedures.” The values represent the mean and S.D. for three measurements each.
| Substrates | LmNcb5or enzyme |
||
|---|---|---|---|
| Km | kcat | kcat/Km | |
| μm | s−1 | μm−1 s−1 | |
| NADH | 3.3 ± 0.2 | 0.2 ± 0.05 | 0.06 |
| Cytochrome c | 12 ± 0.6 | 15 ± 0.8 | 1.25 |
| Potassium ferricyanide | 9 ± 0.7 | 26 ± 1.0 | 2.88 |
| DCIP | 14 ± 0.4 | 9 ± 0.9 | 0.64 |