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. 2012 Aug 8;287(42):35078–35091. doi: 10.1074/jbc.M112.401000

TABLE 1.

Bacterial strains and plasmids

Strain/plasmid Description or genotype Reference or source
Strains
    Top10 E. coli F, mcrA, Δ (mrr-hsdRMS-mcrBC), φ80, lacZΔM15, ΔlacX74, deoR, nupG, recA1, araD139, Δ(ara-leu)7697, galU, galK, repsL(Strr), endA1 Invitrogen
    BL21 E. coli B F dcm ompT hsdS(rB mB) gal [malB+]K-12S) Novagen
    CWG634 E. coli O9a:K; trp his lac rpsL cpsK30; manA; Smr; Tcr Ref. 15
    CWG636 E. coli O8:K; ugd::aacC1 manA; Gmr; Tcr Ref. 15
    CWG1005 K-12 lacZ trp Δ(sbcB-rfb) upp rel rpsL manA Ref. 12
    CWG1006 CWG1005 derivative; waaL::cat; Cmr This study
    CWG1007 CWG636 derivative; ΔwbdB; Gmr; Tcr This study
    CWG1008 CWG636 derivative; ΔwbdC; Gmr; Tcr This study
    CWG1009 CWG634 derivative; ΔwbdB; Smr; Tcr This study
    CWG1010 CWG634 derivative; ΔwbdC; Smr; Tcr This study
    CWG1104 CWG636 derivative; ΔwbdA; Gmr; Tcr This study
    CWG1105 CWG634 derivative; ΔwbdA; Smr; Tcr This study

Plasmids
    pMAL-c2X IPTG-inducible plasmid for expressing cytoplasmic maltose-binding protein fusions; Apr New England Biolabs
    pBADHisA l-Arabinose-inducible plasmid to express N-terminal hexahistidine-tagged constructs; Apr Invitrogen
    pBAD24 l-Arabinose-inducible plasmid; Apr Ref. 23
    pKD3 Source of Cmr resistance cassette Ref. 22
    pKD46 Helper plasmid encoding the Red recombinase genes, γ, β, and exo Ref. 22
    pCP20 Helper plasmid encoding the FLP recombinase Ref. 22
    pWQ492 pBAD24 derivative containing an EcoRI/HindIII fragment encoding WbdAO9a-His10; Apr Ref. 58
    pWQ575 pMAL-c2X derivative containing an XmnI/HindIII fragment encoding WbdCO9a; Apr This study
    pWQ576 pMALc-2X derivative containing an EcoRI/HindIII fragment encoding WbdBO9a; Apr This study
    pWQ577 pMALc-2X derivative containing an EcoRI/HindIII fragment encoding WbdCO8; Apr This study
    pWQ578 pMALc-2X derivative containing an EcoRI/HindIII fragment encoding WbdBO8; Apr This study
    pWQ579 pBAD24 derivative containing an EcoRI/HindIII fragment encoding WbdCO9a; Apr This study
    pWQ580 pBAD24 derivative containing an EcoRI/HindIII fragment encoding WbdCO9a-WbdBO9a; Apr This study
    pWQ581 pBAD24 derivative containing an EcoRI/HindIII fragment encoding WbdCO8; Apr This study
    pWQ582 pBAD24 derivative containing an EcoRI/HindIII fragment encoding WbdCO8-WbdBO8; Apr This study
    pWQ587 pBADHisA derivative containing an XhoI and PvuII fragment encoding WbdAO8; Apr This study
    pWQ588 pBAD24 derivative containing an NcoI/XbaI fragment encoding His10-WbdAO8; Apr This study