Strains |
|
|
Top10 |
E. coli F−, mcrA, Δ (mrr-hsdRMS-mcrBC), φ80, lacZΔM15, ΔlacX74, deoR, nupG, recA1, araD139, Δ(ara-leu)7697, galU, galK, repsL(Strr), endA1
|
Invitrogen |
BL21 |
E. coli B F−
dcm ompT hsdS(rB− mB−) gal [malB+]K-12(λS) |
Novagen |
CWG634 |
E. coli O9a:K−; trp his lac rpsL cpsK30; manA; Smr; Tcr
|
Ref. 15
|
CWG636 |
E. coli O8:K−; ugd::aacC1 manA; Gmr; Tcr
|
Ref. 15
|
CWG1005 |
K-12 lacZ trp Δ(sbcB-rfb) upp rel rpsL manA
|
Ref. 12
|
CWG1006 |
CWG1005 derivative; waaL::cat; Cmr
|
This study |
CWG1007 |
CWG636 derivative; ΔwbdB; Gmr; Tcr
|
This study |
CWG1008 |
CWG636 derivative; ΔwbdC; Gmr; Tcr
|
This study |
CWG1009 |
CWG634 derivative; ΔwbdB; Smr; Tcr
|
This study |
CWG1010 |
CWG634 derivative; ΔwbdC; Smr; Tcr
|
This study |
CWG1104 |
CWG636 derivative; ΔwbdA; Gmr; Tcr
|
This study |
CWG1105 |
CWG634 derivative; ΔwbdA; Smr; Tcr
|
This study |
|
Plasmids |
|
|
pMAL-c2X |
IPTG-inducible plasmid for expressing cytoplasmic maltose-binding protein fusions; Apr
|
New England Biolabs |
pBADHisA |
l-Arabinose-inducible plasmid to express N-terminal hexahistidine-tagged constructs; Apr
|
Invitrogen |
pBAD24 |
l-Arabinose-inducible plasmid; Apr
|
Ref. 23
|
pKD3 |
Source of Cmr resistance cassette |
Ref. 22
|
pKD46 |
Helper plasmid encoding the Red recombinase genes, γ, β, and exo
|
Ref. 22
|
pCP20 |
Helper plasmid encoding the FLP recombinase |
Ref. 22
|
pWQ492 |
pBAD24 derivative containing an EcoRI/HindIII fragment encoding WbdAO9a-His10; Apr
|
Ref. 58
|
pWQ575 |
pMAL-c2X derivative containing an XmnI/HindIII fragment encoding WbdCO9a; Apr
|
This study |
pWQ576 |
pMALc-2X derivative containing an EcoRI/HindIII fragment encoding WbdBO9a; Apr
|
This study |
pWQ577 |
pMALc-2X derivative containing an EcoRI/HindIII fragment encoding WbdCO8; Apr
|
This study |
pWQ578 |
pMALc-2X derivative containing an EcoRI/HindIII fragment encoding WbdBO8; Apr
|
This study |
pWQ579 |
pBAD24 derivative containing an EcoRI/HindIII fragment encoding WbdCO9a; Apr
|
This study |
pWQ580 |
pBAD24 derivative containing an EcoRI/HindIII fragment encoding WbdCO9a-WbdBO9a; Apr
|
This study |
pWQ581 |
pBAD24 derivative containing an EcoRI/HindIII fragment encoding WbdCO8; Apr
|
This study |
pWQ582 |
pBAD24 derivative containing an EcoRI/HindIII fragment encoding WbdCO8-WbdBO8; Apr
|
This study |
pWQ587 |
pBADHisA derivative containing an XhoI and PvuII fragment encoding WbdAO8; Apr
|
This study |
pWQ588 |
pBAD24 derivative containing an NcoI/XbaI fragment encoding His10-WbdAO8; Apr
|
This study |