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. 2012 Aug 24;287(42):35351–35359. doi: 10.1074/jbc.M112.399386

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Mkx-binding sites within the Sox6 locus. Mkx (ATGTT-N₁₁-AACAT)- and Irx (ACAnnTGT)-binding sites were identified in the locus of Sox6. A, EMSAs demonstrate that MT-Mkx-VP16 can physically interact with the Sox6 MBS and IBS sequences in vitro. B, cell-based luciferase assays demonstrate that these sequences are responsive to MT-Mkx-VP16 activation and are dependent upon sequences within Helix III of the homeodomain (MT-Mkx(ΔH3)-VP16) and CD-A (MT-Mkx(ΔCDA)-VP16). An activated form of mouse Irx2 (HA-Irx2-VP16) could only regulate the Sox6 IBS.