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. Author manuscript; available in PMC: 2013 Mar 28.
Published in final edited form as: Cell. 2012 Sep 28;151(1):167–180. doi: 10.1016/j.cell.2012.07.034

Figure 3. CMT3 Is Primarily a CHG Methyltransferase and Predominantly Expressed in Actively Replicating Cells.

Figure 3

(A) CMT3 has activity on both unmethylated and hemi-methylated DNA oligos, but not fully methylated DNA oligos containing methylations at all cytosines. Error bars represent the standard deviation of three replicates.

(B) Bisulfite sequencing of in vitro methylated plasmid DNA.

(C) Hemimethylated dyads at the Ta3 locus were significantly lower than expected (Chi square value: 32.84). Probability of obtaining the observed distribution by random chance is less than 0.1%.

(D) Accumulation of CMT3 in cells with active replication. DAPI staining shows the localization of nuclei (Blue). Actively replicating cells were labeled by 5-ethynul-2′-deoxyuridine (EdU, Red) and CMT3 was immuno-stained by MYC antibody (Green).

See also Figures S1B and Tables S3 and S4.