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. 2012 Oct 15;7(10):e47449. doi: 10.1371/journal.pone.0047449

Figure 2. The radio-sensitivity of glioma cells according to the modulation of microRNA 21 (miR-21).

Figure 2

Anti-miR-21 (100 nM) or negative control (100 nM) was transiently transfected into U373 and U87 cells for loss-of-function. miR-21 (1 µg) or control plasmid (1 µg) was transiently transfected into LN18 and LN 428 cells for gain-of-function. The cells were plated onto 96 well microplates and incubated for a further 48 hours prior to γ-irradiation. After exposure to the indicated level of ionizing irradiation, the surviving cell fraction was measured using a sulforhodamin-B (SRB) assay at 5 days, and the effect of miR-21 modulation is illustrated for each cell line (A–F). Each error bar indicates the standard deviation of three independent experiments.