Figure 3. Summary of Tension (A and E), Stiffness (B and F), pCa₅₀ (C and G), and Cooperativity (D and H).

(A–D) Data obtained in high-IS solutions. (E–H) Data obtained in low-IS solutions [22]. (A, E) Low calcium tension (T _LC) represents the tension at pCa 7. High calcium tension (T _HC) represents the tension at pCa 4.66. Active tension (T _act) is the tension developed on Ca²⁺ activation (T _act = T _HC–T _LC). (B, F) Results of the stiffness study. Tension and stiffness were normalized to Ta. (C, G) pCa₅₀ (Ca²⁺ sensitivity). (D, H) Cooperativity. *: p<0.05.