Table 1. Comparison of HCM- and DCM-related Tm mutations on isomeric tension and cross-bridge kinetics.
| Parameters (unit) | HCM mutants (V95A, D175N and E180G) | DCM mutants (E40K and E54K) |
| T LC (Ta) | All ↑ (2–3 fold) | Both ↓ (∼50%) |
| T HC (Ta) | E180G ↑ (∼17%) | Both ↓ (∼20%) |
| T act (Ta) | V95A and D175N ↓ (∼28%) | Both ↓ (∼20%) |
| Y LC (Ta) | All ↑ (2–2.5 fold) | Both ↓ (∼5%) |
| Y HC (Ta) | E180G ↑ (∼33%) | Both ↓ (∼33%) |
| Y act (Ta) | D175N ↓ (∼33%) | Both ↓ (∼20%) |
| pCa50 | V95A and E180G ↑ | Remain similar in high-IS solution Both ↓ in low-IS solution |
| Hill Factor (Cooperativity) | All ↓ (∼38%) | E54K ↑ (∼20%) |
| K 0 (mM−1) | V95A↓ (∼40%) and E180G ↑ (∼35%) | E40K ↓ (∼70%) |
| K 1 (mM−1) | V95A↓ (∼50%) | E40K ↑ (∼6 fold) E54K ↑ (∼3 fold) |
| K 2 | V95A↓ (∼33%) | E40K ↑ (∼84%) |
| K 4 | All remain similar to WT | E40K ↓ (∼67%) |
| K 5 (mM−1) | V95A and D175N ↓ (∼60%) | E40K ↓ (∼50%) |
| k 2 (s−1) | D175N ↑ (∼30%) | E40K ↑ (∼70%) E54K ↓ (∼30%) |
| k- 2 (s−1) | V95A ↑ (∼60%) | E54K ↓ (∼20%) |
| k 4 (s−1) | V95A ↑ (∼40%) | Both remain similar to WT |
| k -4 (s−1) | All remain similar to WT | E40K ↑ (∼2 fold), E54K ↑ (∼50%) |
Notes: ↑ increase, ↓ decrease; Only significant changes are indicated; All percent change is in comparison to WT. The data on HCM are from earlier publication [17].