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. 2012 Oct 15;7(10):e47381. doi: 10.1371/journal.pone.0047381

Figure 2. Chronic treatment of isolated islets with PIK-75 reduced the glucose-induced insulin secretion.

Figure 2

(A) Pancreatic islets cultured for 48 h with PIK-75 at the indicated concentrations were subjected to immunoblotting using anti-Pi-Akt and Pan-Akt antibodies. (B) Pancreatic islets cultured for 48 h with PIK-75 at the indicated concentrations were stimulated with 16 mM glucose with or without 0.5 µM PIK-75 for 30 min. The amount of secreted insulin was normalized to that from the PIK-75 untreated islets of each group (n = 8 for each group). (C) Freshly isolated islets or those cultured with or without 1 µM PIK-75 for 24, 48 and 72 h were stimulated with 16 mM glucose supplemented with or without 0.5 µM PIK-75 for 30 min. The amount of secreted insulin was normalized to that from the PIK-75 untreated islets of each group (n = 8 for each group). Data are the means ± S.E.M. (*, p<0.01; **, p<0.03).