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. 2012 Oct 15;7(10):e47355. doi: 10.1371/journal.pone.0047355

Figure 10. ILK and DIAP1 coexpressed with Parvin-GFP do not rescue the dissorganized integrin-matrix adhesion sites.

Figure 10

Confocal optical sections acquired from wing imaginal discs of late third instar larvae expressing UAS::Parvin-GFP (green, A–B, A1–A1′; white, A′′–B′′) with both UAS:ILK and UAS:DIAP1 driven by enGal4 in the posterior compartment and probed for LamininA (red, A, A1–A1′; white, A′) or βPS integrin (red, B; white, B′) and DAPI to visualize nuclei (blue, A, A1, A1′′). (A1) cross optical section of the imaginal disc appearing in image A taken in the middle of the wing poutch. Small arrows, closed areas in the posterior and anterior compartment of the wing pouch expressing (right) or not expressing (left) UAS::Parvin-GFP; big arrows, indicate areas with LamininA deposition; big dashed arrows indicate areas without LamininA deposition; arrowheads, focal contact-like structures in the basal side of the anterior compartment and open arrowheads indicate areas in the posterior compartment without integrin accumulation. The anterior part of the wing disc (where Parvin-GFP expression is not induced) serves as an internal control.