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. 2012 Oct 15;7(10):e46287. doi: 10.1371/journal.pone.0046287

Figure 1. Effects of BDNF on RANKL expression in human bone marrow stromal cells (BMSCs).

Figure 1

(A) The dose and time responses to BDNF of RANKL mRNA expression in BMSCs. PCR analysis indicates that BDNF markedly increases RANKL mRNA expression in human BMSCs (B) Western blot analysis shows that BDNF increased RANKL protein expression by human BMSCs in a dose- and time-dependent manner. (C) K252a diminishes BDNF-mediated RANKL secretion in human BMSCs. Stimulation with 25 ng/ml BDNF was performed in the absence or presence of K252a pretreatment (50, 100 nM; 60 min) in BMSCs. K252a without BDNF was used for control stimulation. RANKL levels in supernatants at different time points (0, 12, 24, 48 h) were detected by ELISA.