Figure 5. Antisense inhibition of BDNF inhibits osteoclast formation and RANKL expression in myelomatous bones.

(A) TRAP and H&E staining of the myelomatous bones. Representative osteoclasts are labeled with arrows. Magnification ×200. (B) Quantitative analysis of osteoclasts on trabecular bone surface. Data represent the means ± SEMs of five separate high-power fields (HPFs). (C) Bone engrafts were fetched and cells in bones were obtained by flushing the bones repeatedly with 1 ml of PBS. Expression levels of RANKL and BDNF were measured by western blot Results indicate that RANKL and BDNF protein level in AS-ARH-infiltrated bones (lanes 3, 4) were much lower than those in EV-ARH group (lanes 1, 2). (D) Immunohistochemistry analysis indicates that the number of RANKL-positive cells in bone sections from the AS-ARH group was markedly decreased compared to the EV-ARH group .Magnification ×400.