Table 1. Expression of HP1 and ZNF genes in lamin A/C knock-down clones.

Gene	Cl 26	Cl 27
HP1α	1.88±0.39	1.92±0.22
HP1β	1.19±0.48	1.33±0.22
HP1γ	1.38±0.38	1.58±0.46
ZNF44	2.89±0.65**	2.58±0.54*
ZNF77	2.02±0.28**	2.40±0.58*
ZNF85	2.04±0.24*	2.17±0.67*
ZNF226	1.35±0.42	1.20±0.23
ZNF266	1.63±0.21**	1.56±0.44

Values indicate fold-change in real-time PCR transcripts (upregulation) expressed as mean ± SD calculated from three independent experiments for two stable HeLa clones, Cl 26 and 27 expressing lamin A/C shRNA, compared to a stable clone expressing the control vector.

^* p≤0.05,

^** p≤0.01. Cl 26 and 27 showed 2- and 5-fold reduction in lamin A/C transcripts respectively and ∼90% depletion of lamin A/C protein [ref 20].