Figure 5. Biological functions related to global gene expression changes.

Graphs of biological functions were generated using “focus” genes and the database of gene interactions present within Ingenuity Pathway Analysis software presented according to day 4 EB ranking results. (A) The ten highest-ranked biological functions that were significant at different differentiation time points consisted of processes typically related to development, including morphogenesis and proliferation. (B) In contrast, the ten lowest-ranked biological processes included disease states not typically associated with development.