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. 2012 Oct 15;7(10):e46476. doi: 10.1371/journal.pone.0046476

Figure 1. CRAMP-deficient mice have a thinner layer of mucus in the colon.

Figure 1

(A and B) Mouse colon tissues from a representative colonic segment taken from 129/SvJ (A, wild-type) and Camp−/− (B) mice, fixed by water-free Methanol-Carnoy's fixative, stained with PAS and visualized by light microscopy. Stars indicate thickness of the inner mucus layer. Scale bar indicates 25 µm. (C) The thickness of the inner stratified layer of colonic mucus in µm. The individual values and means are displayed for each group of mice (N = 6 and 5, respectively). The inner mucus layer in Camp−/− mice was thinner by 32.7% (p<0.01) (D) The total amount of colonic mucus expressed as the amount of Alcian blue bound to mg of colonic tissue. The individual values and medians are shown (N = 4 in each group). The amount of mucus in Camp−/− mice was lower by 43.8% compared with the wild-type animals (p<0.05).