Table 1. Virulence factor genes tested by Real time RT-PCR.

Gene	Function	Primers	Reference
16S rRNA	Internal standard gene	F: CTGGTAGTCCACGCCGTAAAC R: CAGGCGGAGTGCTTAATGC	[71]
icaA	Polysaccharide intercellular adhesion/polymeric N-acetyglucosamine production	F: AACAGAGGTAAAGCCAACGCACTC R: CGATAGTATCTGCATCCAAGCAC	[35]
dltD	Esterification of teichoic acids with D-alanine	F: GTGCTGCTGGTGCAGATGTTCAAT R: CTGCTTGACGACGTTCTTTATCG	[31], [36]
atlA	Autolysin	F: TGTCGAAGTATTTGCCGACTTCGC R: TGGAATCCTGCACATCCAGGAAC	[32], [37]
Psmα operon	Phenol soluble modulins α	F: ACCCATGTGAAAGACCTCCTTTGT R: ATGGGTATCATCGCTGGCATC	[52], [72]
SPA	Surface and secreted protein for bacterial aggregation	F: GCGCAACACGATGAAGCTCAACAA R: ACGTTAGCACTTTGGCTTGGATCA	[38]
lrgA	Cell death and lysis	F: CTGGTGCTGTTAAGTTAGGCGAAG R: GGCTGGTACGAAGAGTAAGCCAAT	[53]
sdrD	SD-repeat-containing protein	F : AGTACACAGTGGGAACAGCATC R : TCTGCAGCCTTTGCTTCTTGGTTC	[41], [42]
sspB	Cysteine protease	F: CCAGCAAATTGTTGTTGTGCTAG R: AAGCCAAAGCCGATTCACACTC	[44], [73]
SigB	Gene expression regulator	F: TCAGCGGTTAGTTCATCGCTCACT R: GTCCTTTGAACGGAAGTTTGAAGCC	[46]
AgrA	Gene expression regulator	F: AAGCATGACCCAGTTGGTAACA R:ATCCATCGCTGCAACTTTGTAGA	[74]
RNAIII	Gene expression regulator	F: GCACTGAGTCCAAGGAAACTAACTCT R: AGCCATCCCAACTTAATAACCATGT	[46]
CodY	Gene expression regulator	F: AAAGAAGCGCGCGATAAAGCTG R: TGCGATTAATAGGCCTTCCGTACC	[55], [56]
ebpS	Surface protein	F: TTTCCGGTGAACCTGAACCGTAGT R: ACAGCAACAACAACGTCAAGGTGG	[42]
cidA	Cell death and lysis	F: AGCGTAATTTCGGAAGCAACATCC R: TACCGCTAACTTGGGTAGAAGACG	[54]
Hla	Alpha-Toxin	F: CTGAAGGCCAGGCTAAACCACTTT R: GAACGAAAGGTACCATTGCTGGTCA	[51]