Figure 3. NaOH-SDS treatment lysed gram-negative bacteria within 5 min.

(A) The mid-log phase culture of 4 gram-negative bacterial strains (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii) and Enterococcus faecalis (gram-positive bacteria used as control) were treated with the NaOH-SDS solution for the indicated time. The numbers of bacteria that were measured by the UF-1000i were converted into a percentage relative to the control that was treated with an equal volume of PBS. Each symbol represents the average of 3 independent experiments, and the error bars represent the standard deviation of the mean. (B) Representative BACT scattergrams of the NaOH-SDS-treated bacterial cultures that were incubated for 1 or 5 min, or control (0 min).