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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: J Biol Chem. 2008 Nov 18;284(3):1505–1513. doi: 10.1074/jbc.M805113200

FIGURE 2. Effect of CaRRE1 replacement by an hnRNP L-high affinity CA repeat element in CaMKIV-regulated splicing.

FIGURE 2

Upper, diagram of the exons (boxes) and introns (horizontal lines) of the splicing reporter mini-gene DUP175ST and its CaRRE1 in the upstream 3′-splice site of STREX (heavy line). In reporters L1 or L1m, the CaRRE1 was replaced with the hnRNP L high affinity CA repeats (L1) or low affinity sequence (L1m). Arrowheads, locations of PCR primers. Lower, an agarose gel of RT-PCR products from RNA samples of HEK293T cells co-transfected with each of the above splicing reporters and kinase-dead mutant (IVm) or constitutively active CaMKIV (IV), with average percentages (±S.D.) of exon inclusion under each lane, exon-included or -excluded products indicated to the right, and molecular size makers (M) to the indicated to the left. NC, PCR negative control. *, likely product from pre-mRNA. **, a cryptic 3′-splice product from 65 nt downstream in the middle exon.