Skip to main content
. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Nat Med. 2012 May;18(5):799–806. doi: 10.1038/nm.2729

Figure 5. E. coli-O21:H+ activates the Naip5-Nlrc4 inflammasome.

Figure 5

(a) Motility agar was inoculated with wild-type, motile S. Typhimurium LT2, flagellin-deficient S. Typhimurium ΔfliCΔfljB or E. coli-O21:H+ and incubated at 37 °C overnight. Representative images of the diffusion of motile bacteria are shown.

(b) Retroviral constructs expressing fliC from E. coli-O21:H+ or flaA from L. pneumophila followed by an IRES-GFP element or GFP alone were transduced into wild-type, Naip5−/−, or Nlrc4−/− macrophages, and the percentage of GFP-positive cells was enumerated by flow cytometry 4 d post-transduction.

(c) The percentage of cells expressing GFP from the retroviral lethality assay in (b) is indicated and charted.

Data are representative of three independent experiments.